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醫(yī)學論文范文:Toll樣受體2對角質(zhì)形成細胞分泌IL8的影響
【摘要】 目的:觀察白念珠菌刺激人角質(zhì)形成細胞后IL8的變化以及Toll樣受體2(Tolllike receptor 2，TLR2)對IL8分泌的影響，探討角質(zhì)形成細胞在皮膚免疫系統(tǒng)中的作用。方法：白念珠菌分別與抗TLR2抗體預(yù)處理前后的角質(zhì)形成細胞孵育，ELISA方法檢測不同時間培養(yǎng)上清液IL8的水平。結(jié)果：白念珠菌可引起IL8明顯升高(P<0.01)，3h開始，至24h達到高峰;中和TLR2受體后，雖然引起IL8升高,但無統(tǒng)計學意義(P>0.05)。結(jié)論：白念珠菌可以刺激角質(zhì)形成細胞分泌IL8，TLR2在角質(zhì)形成細胞IL8表達中的作用尚難確定。
【關(guān)鍵詞】 白念珠菌 Toll樣受體2 IL 8 角質(zhì)形成細胞 天然免疫
Effects of Tolllike Receptor2 on Interleukin8 Secretion of Keratinocytes HUANG Guangrong1, FAN Li1, DUAN Yingchun1, DUAN Dejian2^ (1Department of Obstetrics and Gynecology,Renmin Hospital;2Department of Dermatovenereology,Taihe Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China)
Abstract: Objective To observe the change of Interleukin8(IL8) in keratinocytes induced with Candida Albicans and the effect of Tolllike receptor2(TLR2) on IL8 secretion,to explore the role of keratinocytes in skin immune system. Methods Candida Albicans were cultured on keratinocytes treated before and after antiTLR2 monoantibody(mAb),respectively.The levels of IL8 in culture supernatant were determined with ELISA at different time points. Results The IL8 levels were significantly increased after induced with Candida Albicans(P<0.01),the significant increase was observed at 3 h and reached the peak at 24 h;the increase of IL8 levels had no significant difference after TLR2 was neutralized with antiTLR2 mAb(P>0.05). Conclusion The secretion of IL8 in keratinocytes could be induced with Candida albicans,the function of TLR2 is uncertain in expression of IL8 in keratinocytes醫(yī).學全.在.線網(wǎng)站zxtf.net.cn.
Key words:Candida albicans;Tolllike receptor2;Interleukin8;Keratinocytes;Innate immunity
念珠菌在人體表面的分布廣泛而常見，而白念珠菌是引起念珠菌病的主要致病菌種。在去除表皮的實驗說明表皮角質(zhì)形成細胞在抵抗念珠菌感染中起重要作用。皮膚粘膜念珠菌病常表現(xiàn)為病原菌寄生部位的角層下膿皰或角層內(nèi)大量中性粒細胞聚集，但是否與細胞表達的趨化因子IL8有關(guān)，目前還不太清楚[1]。Toll樣受體2(Tolllike receptor 2，TLR2)是表達于角質(zhì)形成細胞并參與真菌產(chǎn)物識別的天然受體蛋白，其與IL8的關(guān)系亦研究不多，故本研究旨在探討TLR2對角質(zhì)形成細胞分泌IL8的影響。
