通過(guò)核酸標(biāo)記技術(shù)可將細(xì)胞因子cDNA作為基因探釗檢測(cè)細(xì)胞內(nèi)細(xì)胞因子基因組DNA或mRNA。主要有以下幾種方法：
1.應(yīng)用同位素（或非同位素)標(biāo)記的cDNA探針，檢測(cè)經(jīng)Northern blot后細(xì)胞因子mRNA水平或采用打點(diǎn)雜交法。
2.應(yīng)用標(biāo)記cDNA探釗與細(xì)胞或組織切片進(jìn)行原位雜交，然后進(jìn)行放射自顯影。
3.細(xì)胞因子mRNA經(jīng)反轉(zhuǎn)錄為cDNA，用特異性細(xì)胞因子引物經(jīng)聚合酶鏈反應(yīng)（PCR)擴(kuò)增細(xì)胞因子cDNA,Southern blot后用標(biāo)記探針檢測(cè)特異細(xì)胞因子DNA水平。
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