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醫(yī)學免費論文:異常黑膽質證載體大鼠模型的建立及其免疫、內分泌紊亂狀態(tài)的研究
【摘要】 目的建立維吾爾醫(yī)異常黑膽質證載體大鼠模型，并探討其免疫、內分泌的紊亂狀態(tài)。方法雄性Wistar大鼠20只, 穩(wěn)定飼養(yǎng)3 d后隨機分為正常對照組(10只)和模型組(10只),其中模型組根據維吾爾醫(yī)學理論，采用干寒飼養(yǎng)環(huán)境、干寒屬性的飼料、慢性間斷足底電刺激、強迫游泳、制動等多因素復合作用3周，建立維吾爾醫(yī)異常黑膽質證載體大鼠模型，動態(tài)觀察模型組大鼠一般狀態(tài)、體重、飲食量、飲水量和舌苔的變化;檢測外周血白細胞介素1β(IL1β)、白細胞介素6(IL6)、腫瘤壞死因子α(TNFα)、促腎上腺皮質激素(ACTH)和皮質醇(CORT)等免疫、內分泌指標的變化。結果與正常對照組相比，模型組動物的一般狀態(tài)與異常黑膽質證候特征相一致。第11天至第21天體重增長速度慢，自第7天至第21天飲食量、飲水量增多，差異有統計學意義(P<0.01)。與正常對照組相比，模型組動物血清ACTH、CORT、IL1β水平均降低，差異有統計學意義(P<0.05~0.01),IL6水平降低，但差異無統計學意義(P>0.05),TNFα水平升高,差異有統計學意義(P<0.05)。結論異常黑膽質證載體大鼠模型的一般狀態(tài)、體重、飲食量、飲水量的變化與臨床患者以及小鼠動物模型中異常黑膽質證相似，并且其免疫、內分泌處于紊亂狀態(tài)。
【關鍵詞】 異常黑膽質; 維吾爾醫(yī)學; 動物模型
Establishment of abnormal savda syndrome rat model and study on its disordered immuneendocrine network
ZHANG Youhui, Halmurat·Upur, Yusup·Tursun, et al醫(yī).學.全.在.線zxtf.net.cn
(Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830011, China)
Abstract: ObjectiveTo establish a rat model of abnormal savda syndrome in Uygur medicine and investigate its disordered immuneendocrine network. MethodsMale Wistar rats (n=20)， were randomly divided into control group (n=10) and model group (n=10) after stable feeding 3 d. According to the theory of Uygur medicine, model group rats were fed with coldnatured diet under cool dry environment, stimulated with chronics and interrupted electric foot shocks, enforced swimming and controlled movement to establish abnormal savda syndrome rat model for three weeks, rats′ behavior, body weight, daily food intake, daily water intake, state of tongue were observed. The concentrations of immuneendocrine related indexes including Interleukin1β (IL1β), Interleukin6 (IL6), Tumor necrosis factorα (TNFα), Adrenocorticotropic Hormone (ACTH) and Cortisol (CORT) in serum were measured by enzyme linked immunosorbent assay (ELISA) method. ResultsCompared with the control group, the model group developed abnormal savda syndrome like slow weight gaining, more diet, more drink. Compared with the control group, the serum level of ACTH， CORT， IL1β in model group decreased，and the differences had statistical significance (P<0.05~ 0.01); the serum level of IL6 in model group decreased，but the differences had no statistical significance (P>0.05); the serum level of TNFα increased, and the differences had statistical significance (P<0.05). ConclusionThe changes of body weight, daily food intake, daily water intake of abnormal savda syndrome rat model are similar to the feature of human abnormal savda syndrome and the abnormal savda syndrome model of ICR mice. Abnormal savda syndrome rat model developes disordered immuneendocrine network.
Key words: abnormal savda; Uygur medicine; animal model
維吾爾醫(yī)學認為異常黑膽質是在各種內外因素的作用下，體液質平衡紊亂，體液質被燃燒，繼而沉淀的最終病理產物或表現形式。長期受到慢性刺激、進食干寒屬性的飼料、處于干寒的環(huán)境是異常黑膽質證發(fā)生的主要起因[1-2]。隨著維吾爾醫(yī)學的深入研究,需要建立具有維吾爾醫(yī)學證候特征的大鼠模型,以便運用現代科學手段研究維吾爾醫(yī)學。本研究以維吾爾醫(yī)學理論為指導，采用干寒飼養(yǎng)環(huán)境、干寒屬性的飼料、慢性間斷足底電刺激、強迫游泳和制動等多因素復合作用3周，建立維吾爾醫(yī)異常黑膽質證載體大鼠模型，并檢測其外周血白細胞介素1β(IL1β)、白細胞介素6(IL6)、腫瘤壞死因子α(TNFα)、促腎上腺皮質激素(ACTH)和皮質醇(CORT)等免疫、內分泌指標的變化，探討其免疫、內分泌的紊亂狀態(tài)。
1 材料與方法
