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醫(yī)學論文范文:人腎透明細胞癌組織中增殖細胞核抗原表達與腎癌的關(guān)系
【摘要】 目的 通過檢測人腎透明細胞癌組織中增殖細胞核抗原(PCNA)表達的變化，研究其表達與腎癌的關(guān)系。方法 標本離體后分成腎透明細胞癌組、癌旁組織組、正常腎組織組，均用10%的甲醛固定48 h，常規(guī)石蠟包埋、切片5 μm，同時應用免疫組織化學方法進行觀察研究。結(jié)果 PCNA免疫組化染色：正常腎組織內(nèi)的細胞核有弱陽性表達，癌旁陽性表達增加，高于正常腎組織，腎癌組織陽性表達明顯增強， 明顯高于癌旁組織，兩兩比較差異均有統(tǒng)計學意義(P<0.01)。腎癌4個期之間比較差異無統(tǒng)計學意義(P>0.05)。結(jié)論 PCNA在正常組、癌旁組、癌組織組的表達呈明顯加強趨勢，癌組織細胞增殖旺盛，是腫瘤形成的重要機制。
【關(guān)鍵詞】 人腎透明細胞癌;增殖細胞核抗原
The expression of proliferating cell nuclear antigen (PCNA ) in the human clear cell renal cell carcinoma ZHANG Jianyong^, LIANG Liang, DONG Xiaoju,et al.^Department of Urology,The Second Hospital of Shijiazhuang,Hebei,Shijianzhuang 050051,China
【Abstract】 Objective To detect the expression of proliferating cell nuclear antigen (PCNA ) in the human clear cell renal cell carcinoma(RCCC) tissues in order to explore the correlations between the antigen expression and RCCC.Methods The samples were divided into three groups: RCCC group, paracancerous tissue group and normal tissue group. All the samples were fixed in formalin for 48h, embedded in paraffin wax, sectioned to 5μm thick slice, and observed by immunohistochemical method. Results PCNA immunohistochemical staining showed that the expression of PCNA was weakly positive in the nucleus of the normal renal tissue. The expression was slightly higher in paracancerous tissue than that in normal renal tissue. The expression was significantly increased in RCCC tissue ,which was significantly higher than that in paracancerous tissue (P<0.01), but there were no significant differences among 4 stages (P>0.05). Conclusion the expression of PCNA in RCCC tissue is higher than that in para-cancerous tissue, which is the lowest in normal renal tissue. It may be one of the important mechanisms in tumor formation.
【Key words】 human renal clear cell carcinoma; proliferating cell nuclear antigen醫(yī).學全.在.線網(wǎng)站zxtf.net.cn
細胞增殖與凋亡是腫瘤形成的重要理論基礎(chǔ)，是組織正常生長，行使功能的兩個基本要素。二者互相制約，處于動態(tài)平衡之中，組織細胞受損或增殖過度時，通過凋亡清除受損及異常過多的細胞，防止組織進一步損傷，而凋亡或其他原因丟失的死亡細胞則由增殖來補充，以保持正常的細胞數(shù)目及體積。這些精細調(diào)節(jié)的失衡會導致疾病的發(fā)生[1]。組織細胞凋亡減少增殖過度就會導致腫瘤的發(fā)生，細胞增殖的研究是腫瘤形成探討的重要方面。
1 材料與方法
1.1 材料 標本均來自2006年1月至2008年1月石家莊市第二醫(yī)院和解放軍260醫(yī)院泌尿外科，其中腎透明細胞癌30例(Ⅰ期7例,Ⅱ期10例,Ⅲ期8例,Ⅳ期5例)、癌旁組織(距腫瘤組織邊緣2.0 cm以上[2])30例(Ⅰ期7例,Ⅱ期10例,Ⅲ期8例,Ⅳ期5例)、意外傷亡正常腎組織10例(癌旁腎組織，和腎透明細胞癌取自同一患者)。腎透明細胞癌及癌旁組織標本來源的患者60例，男40例，女20例;年齡52～78歲，平均年齡64歲。正常腎組織標本來源的意外傷害者7例，男5例，女2例;年齡19～48歲，平均年齡35歲。
